abstract：:Because of its elevated cellulolytic activity, the filamentous fungus Trichoderma harzianum has a considerable potential in biomass hydrolysis applications. Trichoderma harzianum cellobiohydrolase I (ThCBHI), an exoglucanase, is an important enzyme in the process of cellulose degradation. Here, we report an easy singl...

journal_title：Journal of microbiology and biotechnology

authors： Colussi F,Serpa V,Delabona Pda S,Manzine LR,Voltatodio ML,Alves R,Mello BL,Pereira N Jr,Farinas CS,Golubev AM,Santos MA,Polikarpov I

更新日期：2011-08-01 00:00:00

abstract：:Many studies have shown that the steamed root of Rehmannia glutinosa (SRG), which is widely used in the treatment of various neurodegenerative diseases in the context of Korean traditional medicine, is effective for improving cognitive and memory impairments. The purpose of this study was to examine whether SRG extrac...

journal_title：Journal of microbiology and biotechnology

authors： Lee B,Shim I,Lee H,Hahm DH

更新日期：2011-08-01 00:00:00

abstract：:Streptococcus pneumoniae is a major cause of invasive infection in young infants and older adults. There are currently 90 capsular serotypes identified and 23 serotypes (1, 2, 3, 4, 5, 6B, 7F, 8, 9N, 9V, 10A, 11A, 12F, 14, 15B, 17F, 18C, 19F, 19A, 20, 22F, 23F, and 33F) are responsible for about 90% of invasive diseas...

journal_title：Journal of microbiology and biotechnology

authors： Jung SJ,Seo ES,Yun SI,Minh BN,Jin SD,Ryu HJ,Kim D

更新日期：2011-07-01 00:00:00

abstract：:The diversity elucidation by amplified ribosomal DNA restriction analysis and 16S rDNA sequencing of 96 associative diazotrophs, isolated from the feeder roots of tea on enriched nitrogen-free semisolid media, revealed the predominance of Gram-positive over Gram-negative bacteria within the Kangra valley in Himachal P...

journal_title：Journal of microbiology and biotechnology

authors： Gulati A,Sood S,Rahi P,Thakur R,Chauhan S,Chawla I

更新日期：2011-06-01 00:00:00

abstract：:A commercially important alkaline protease, produced by Bacillus sp. RRM1 isolated from the red seaweed Kappaphycus alvarezii (Doty) Doty ex Silva, was first recognized and characterized in the present study. Identification of the isolated bacterium was done using both biochemical characterization as well as 16S rRNA ...

journal_title：Journal of microbiology and biotechnology

authors： Rajkumar R,Kothilmozhian J,Ramasamy R

更新日期：2011-06-01 00:00:00

abstract：:The infection of pandemic influenza viruses such as swine flu (H1N1) and avian flu viruses to the host cells is related to the following two factors: First, the surface protein such as HA (hemagglutinin) and NA (neuraminidase) of the influenza virus. Second, the specific structure of the oligosaccharide [sialic acid(a...

journal_title：Journal of microbiology and biotechnology

authors： Yoo ES

更新日期：2011-05-01 00:00:00

abstract：:The correlation between alcoholic fermentation rate, measured as carbon dioxide (CO2) evolution, and the rate of hydrogen sulfide (H2S) formation during wine production was investigated. Both rates and the resulting concentration peaks in fermentor headspace H2S were directly impacted by yeast assimilable nitrogenous ...

journal_title：Journal of microbiology and biotechnology

authors： Butzke CE,Park SK

更新日期：2011-05-01 00:00:00

abstract：:To find alternative genetic resources for D-serine dehydratase (E.C. 4.3.1.18, dsdA) mediating the deamination of D-serine into pyruvate, metagenomic libraries were screened. The chromosomal dsdA gene of a wild-type Escherichia coli W3110 strain was disrupted by inserting the tetracycline resistance gene (tet), using ...

journal_title：Journal of microbiology and biotechnology

authors： Lim MY,Lee HJ,Kim P

更新日期：2011-04-01 00:00:00

abstract：:An endocellulase-free multienzyme complex was produced by a thermophilic anaerobic bacterium, Thermoanaerobacterium thermosaccharolyticum strain NOI-1, when grown on xylan. The temperature and pH optima for growth were 60 degrees C and 6.0, respectively. The bacterial cells were found to adhere to insoluble xylan and ...

journal_title：Journal of microbiology and biotechnology

authors： Chimtong S,Tachaapaikoon C,Pason P,Kyu KL,Kosugi A,Mori Y,Ratanakhanokchai K

更新日期：2011-03-01 00:00:00

abstract：:The study objective was to prepare biodegradable branched dextran microspheres encapsulated with His-tagged interferon-alpha (BDM-hIFN-alpha) and evaluate its activity in vitro and in vivo. The glycidyl methacrylate derivatized dextrans (Dex-GMA) as precursor was primarily synthesized by substituting hydroxyl groups o...

journal_title：Journal of microbiology and biotechnology

authors： Hong H,Jo JR,Yeon JH,Hong JT,Jung KH,Yoo SK,Jang BC

更新日期：2011-02-01 00:00:00

abstract：:A new versatile acrylonitrile-bioconverting strain isolated from a petroleum-contaminated sludge sample and identified as Rhodococcus ruber AKSH-84 was used for optimization of medium and biotransformation conditions for nitrilase activity to produce acrylic acid. A simple and rapid HPLC protocol was optimized for qua...

journal_title：Journal of microbiology and biotechnology

authors： Kamal A,Kumar MS,Kumar CG,Shaik T

更新日期：2011-01-01 00:00:00

abstract：:In this study, we investigated the feasibility of producing bioethanol from the hydrolysate of rape stem. Specifically, the most ideal yeast strain was screened, and the microaeration was performed by surface aeration on a liquid medium surface. Among the yeast strains examined, Pichia stipitis CBS 7126 displayed the ...

journal_title：Journal of microbiology and biotechnology

authors： Yeon JH,Lee SE,Choi WY,Choi WS,Kim IC,Lee HY,Jung KH

更新日期：2011-01-01 00:00:00

abstract：:The first gene (alpha-gal1) encoding an extracellular alpha-Dgalactosidase from the thermophilic fungus Talaromyces emersonii was cloned and characterized. The alpha-gal1 gene consisted of an open reading frame of 1,792 base pairs interrupted by six introns that encoded a mature protein of 452 amino acids, including a...

journal_title：Journal of microbiology and biotechnology

authors： Simila J,Gernig A,Murray P,Fernandes S,Tuohy MG

更新日期：2010-12-01 00:00:00

abstract：:A phosphate-solubilizing bacterial strain isolated from Hippophae rhamnoides rhizosphere was identified as Rahnella sp. based on its phenotypic features and 16S rRNA gene sequence. The bacterial strain showed the growth characteristics of a cold-adapted psychrotroph, with the multiple plant growth-promoting traits of ...

journal_title：Journal of microbiology and biotechnology

authors： Vyas P,Joshi R,Sharma KC,Rahi P,Gulati A,Gulati A

更新日期：2010-12-01 00:00:00

abstract：:We constructed an efficient T vector pTQEST216T that employed an engineered esterase as an indicator for direct cloning of PCR products. After ligation of the XcmI-digested vector with PCR products, this cloning system could easily discriminate positive clones due to insertional inactivation of the esterase reporter. ...

journal_title：Journal of microbiology and biotechnology

authors： Lim HD,Cheong DE,Shin HJ,Kim GJ

更新日期：2010-11-01 00:00:00

abstract：:Glutathione in beer works as the main antioxidant compounds which correlates with beer flavor stability. High residual sugars in beer contribute to major non-volatile components which correlate to high caloric content. In this work, Saccharomyces cerevisiae GSH1 gene encoding glutamylcysteine synthetase and Scharomyco...

journal_title：Journal of microbiology and biotechnology

authors： Wang J,Wang ZY,He XP,Zhang BR

更新日期：2010-11-01 00:00:00

abstract：:Scientific research regarding the use of live bacterial cells for therapeutic purposes has been rapidly growing over the years and has generated considerable interest to scientists and health professionals. Probiotics are defined as essential live microorganisms which, when administered in adequate amounts, confer a h...

journal_title：Journal of microbiology and biotechnology

authors： Islam MA,Yun CH,Choi YJ,Cho CS

更新日期：2010-10-01 00:00:00

abstract：:Rhodococcus sp. JDC-11, capable of utilizing di-n-butyl phthalate (DBP) as the sole source of carbon and energy, was isolated from sewage sludge and confirmed mainly based on 16S rRNA gene sequence analysis. The optimum pH, temperature, and agitation rate for DBP degradation by Rhodococcus sp. JDC-11 was 8.0, 30 degre...

journal_title：Journal of microbiology and biotechnology

authors： Jin DC,Liang RX,Dai QY,Zhang RY,Wu XL,Chao WL

更新日期：2010-10-01 00:00:00

abstract：:Isozyme and protein electrophoresis data from mycelial extracts of 27 isolates of Trichoderma harzianum, 10 isolates of T. aureoviride and 10 isolates of T. longibrachiatum from Southern Peninsular Malaysia were investigated. The eight enzyme and a single protein pattern systems were analyzed. Three isozyme and total ...

journal_title：Journal of microbiology and biotechnology

authors： Siddiquee S,Tan SG,Yusof UK

更新日期：2010-09-01 00:00:00

abstract：:In this study, the identity and distribution of plants and the structure of their associated rhizobacterial communities were examined in an oil-contaminated site. The number of plant species that formed a community or were scattered was 24. The species living in soil highly contaminated with total petroleum hydrocarbo...

journal_title：Journal of microbiology and biotechnology

authors： Lee EH,Cho KS,Kim J

更新日期：2010-09-01 00:00:00

abstract：:In an ongoing investigation of compounds from natural products that exhibit anti-aging properties, hydroxyhibiscone A (1), a new furanosesquiterpenoid, together with hibiscone D (2), was isolated from the root bark of Hibiscus syriacus. Utilizing UV, IR, NMR, and MS spectroscopic analyses, these chemical structures we...

journal_title：Journal of microbiology and biotechnology

authors： Ryoo IJ,Yun BS,Lee IK,Kim YH,Lee IS,Ahn JS,Bae K,Yoo ID

更新日期：2010-08-01 00:00:00

abstract：:A cDNA encoding cysteine protease inhibitor (CPI) was isolated from the cDNA library of clamworm Perinereis aibuhitensis Grube. The deduced amino acid sequence analysis showed that the protein had 51%, 48%, and 48% identity with Zgc:153129 from Danio rerio, cystatin B from Theromyzon tessulatum and ChainA, stefin B te...

journal_title：Journal of microbiology and biotechnology

authors： Li S,Guo D,Zhao B,Ye J,Tian J,Ren W,Ju Y,Cui P,Li R

更新日期：2010-08-01 00:00:00

abstract：:With emphasis on thermal behavior in presence of different pH conditions and salts, the kinetic and thermodynamic parameters of purified polygalacturonase (PG) of E. carotovora subsp. carotovora (Ecc) BR1 were studied since characterization of an enzyme is significant in the context of burgeoning biotechnological appl...

journal_title：Journal of microbiology and biotechnology

authors： Maisuria VB,Patel VA,Nerurkar AS

更新日期：2010-07-01 00:00:00

abstract：:In order to establish an axenic (bacteria-free) culture of Microcystis aeruginosa NIER 10039 isolated from a Korean reservoir, the culture was subjected to sequential treatment, including ultrasonication, washing, and addition of antibiotics. Three broad-spectrum antibiotics, namely, kanamycin, ampicillin, and imipene...

journal_title：Journal of microbiology and biotechnology

authors： Han AW,Oh KH,Jheong WH,Cho YC

更新日期：2010-07-01 00:00:00

abstract：:Pseudomonas putida JM 37 metabolized glyoxylate at a specific rate of 55 g/g dry biomass/day. In order to investigate their role, three genes encoding enzymes that potentially convert glyoxylate were disrupted, namely tartronate semialdehyde synthase (gcl), malate synthase (glcB) and isocitrate lyase (aceA). Strains w...

journal_title：Journal of microbiology and biotechnology

authors： Li XZ,Klebensberger J,Rosche B

更新日期：2010-06-01 00:00:00

abstract：:Bacterial enoyl-ACP reductase (FabI) has been demonstrated to be a novel antibacterial target. In the course of our screening for FabI inhibitors we isolated two methyl-branched fatty acids from Streptomyces sp. A251. They were identified as 14-methyl-9(Z)-pentadecenoic acid and 15-methyl-9(Z)-hexadecenoic acid by MS ...

journal_title：Journal of microbiology and biotechnology

authors： Zheng CJ,Sohn MJ,Chi SW,Kim WG

更新日期：2010-05-01 00:00:00

abstract：:The effects of two different sugars (glucose and xylose) on the expression levels and patterns of xylose reductase (xyl1), xylitol dehydrogenase (xyl2) and xylulokinase (xyl3) genes were analyzed using Pichia stipitis. A significant increase in mRNA levels of xyl1 was observed after 6 hours growth in culture condition...

journal_title：Journal of microbiology and biotechnology

authors： Han JH,Park JY,Kang HW,Choi GW,Chung BW,Min J

更新日期：2010-05-01 00:00:00

abstract：:An antiproliferative ribonuclease with a new N-terminal sequence was purified from fruiting bodies of the edible wild mushroom Russula delica in this study. This novel ribonuclease was unadsorbed on DEAE-cellulose, but absorbed on SP-Sepharose and Q-Sepharose. It had a molecular mass of 14 kDa as judged by fast protei...

journal_title：Journal of microbiology and biotechnology

authors： Zhao S,Zhao Y,Li S,Zhang G,Wang H,Ng TB

更新日期：2010-04-01 00:00:00

abstract：:An efficient and simple fermentation process was developed for the production of gamma-amminobutyric acid (GABA) by Lactobacillus sakei B2-16. When the L. sakei B2-16 was cultivated in the rice bran extracts medium containing 4% sucrose, 1% yeast extract and 12% monosodium glutamate, the maximum GABA concentration rea...

journal_title：Journal of microbiology and biotechnology

authors： Kook MC,Seo MJ,Cheigh CI,Pyun YR,Cho SC,Park H

更新日期：2010-04-01 00:00:00

abstract：:Gene delivery that provides targeted delivery of therapeutic genes to the cells of a lesion enhances therapeutic efficacy and reduces toxic side effects. This process is especially important in cancer therapy when it is advantageous to avoid unwanted damage to healthy, normal cells. Incorporating cancer-specific ligan...

journal_title：Journal of microbiology and biotechnology

authors： Tu Y,Kim JS

更新日期：2010-04-01 00:00:00

abstract：:A series of thiobarbituric acid derivatives were constructed and evaluated for inhibitory activity on hepatitis C virus NS5B polymerase. In biochemical assays using purified viral polymerase and RNA template, the IC(50) value was improved to 0.41 microM from the original compound's 1.7 microM value. In HCV subgenomic ...

journal_title：Journal of microbiology and biotechnology

authors： Lee JH,Lee S,Park MY,Ha HJ,Myung H

更新日期：2010-03-01 00:00:00

abstract：:Archaebacteria Sulfolobus acidocaldarius contains the highly thermophilic cytochrome P450 enzyme (CYP119). CYP119 possesses stable enzymatic activity at up to 85 degrees C. However, this enzyme is still considered as an orphan P450 without known physiological function with endogenous or xenobiotic substrates. We chara...

journal_title：Journal of microbiology and biotechnology

authors： Lim YR,Eun CY,Park HG,Han S,Han JS,Cho KS,Chun YJ,Kim D

更新日期：2010-03-01 00:00:00

abstract：:Evaluation of the primary etiologic agents that cause aseptic meningitis outbreaks may provide valuable information regarding the prevention and management of aseptic meningitis. In Korea, an outbreak of aseptic meningitis caused by echovirus type 30 (E30) occurred from May to October in 2008. In order to determine th...

journal_title：Journal of microbiology and biotechnology

authors： Choi YJ,Park KS,Baek KA,Jung EH,Nam HS,Kim YB,Park JS

更新日期：2010-03-01 00:00:00

abstract：:A Pseudomonas fluorescens strain was isolated and showed antagonistic activity against phytopathogenic fungi and found to possess a gene responsible for production of antibiotic 2, 4-diacetylphloroglucinol. For the extension of biocontrol range, a gene for an Androctonus australis Hector insect toxin 1 (AaHIT1), one o...

journal_title：Journal of microbiology and biotechnology

authors： Lu W,Zhang W,Bai Y,Fu Y,Chen J,Geng X,Wang Y,Xiao M

更新日期：2010-02-01 00:00:00

abstract：:Among human antimicrobial peptides (hAMPs), DCD-1L has a broad spectrum of antimicrobial activity over a wide pH range and in high salt concentrations. It offers a promising alternative to conventional antibiotics. The 458-bp-long dermcidin cDNA was amplified by PCR using a human fetal cDNA library as a template. The ...

journal_title：Journal of microbiology and biotechnology

authors： Hong I,Kim YS,Choi SG

更新日期：2010-02-01 00:00:00

abstract：:The purpose of this study was to investigate the relationship between the global regulatory mechanism known as quorum sensing and expression of virulence factors in Escherichia coli O157:H7. A non-polar luxS deletion was introduced into the chromosome of strain CI03J, a human clinical isolate from South Korea, to crea...

journal_title：Journal of microbiology and biotechnology

authors： Kim JC,Yoon JW,Kim JB,Oh KH,Park MS,Lee BK,Cho SH

更新日期：2010-02-01 00:00:00

abstract：:The contribution of a type II restriction-modification system (R-M system) to genome integrity and cell viability was investigated. We established experimental conditions which enabled the achievement of hemimethylated and unmethylated states for the specific bases of the recognition sequences of the host's DNA. To ac...

journal_title：Journal of microbiology and biotechnology

authors： Katna A,Boratynski R,Furmanek-Blaszk B,Zolcinska N,Sektas M

更新日期：2010-01-01 00:00:00

abstract：:Efficient L-lactic acid production from Jerusalem artichoke tubers by Lactobacillus casei G-02 using simultaneous saccharification and fermentation (SSF) in fed-batch culture is demonstrated. The kinetic analysis in the SSF signified that the inulinase activity was subjected to product inhibition, while the fermentati...

journal_title：Journal of microbiology and biotechnology

authors： Ge XY,Qian H,Zhang WG

更新日期：2010-01-01 00:00:00

abstract：:In biological wastewater treatment high lipid concentration is known to inhibit microorganisms and cause active biomass flotation. To reduce lipid inhibition, a two-phase anaerobic system, consisting of an anaerobic sequencing batch reactor (ASBR) and an upflow anaerobic sludge blanket (UASB) reactor, was applied to s...

journal_title：Journal of microbiology and biotechnology

authors： Kim I,Kim SH,Shin HS,Jung JY

更新日期：2010-01-01 00:00:00

abstract：:The thermophile Bacillus fordii MH602 was screened for stereospecifically hydrolyzing DL-5-substituted hydantoins to L-alpha-amino acids. Since the reaction at higher temperature, the advantageous for enhancement of substrate solubility and for racemization of DL-5-substituted hydantoins during the conversion were ach...

journal_title：Journal of microbiology and biotechnology

authors： Mei YZ,Wan YM,He BF,Ying HJ,Ouyang PK

更新日期：2009-12-01 00:00:00